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This range can be obtained pictorially from Fig. 2 for the specific serial dilution experiment (i.e., α = 10, α p = 1, d plate = 10cm), for other serial dilution parameters the figure RE n ^ must be redrawn (since k is a function of α, α p, d colony, d plate — see Eq. — and δ ^ n ^ is a function of k through Eq. As the name implies, serial dilutions of a sample are spread onto the surfaces of agar plates. MaterialsTapwater or pond water sample Nutrient agar plates Dilution water blanks (9 mL) Pipettes (1 mL, sterile) Pipette bulb or mechanical pump Glass spreaders Alcohol (in a beaker) Vortex mixer Marking pen Methods 1. Procedure - First, we need to do a serial dilution to make solutions with different molarity. Use 0.8M n-butanol solutions and dilute to precisely three quarters the concentration and repeat, and so on until eight concentrations have been used. We will use Distilled water to dilute each solution.
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Experiment 16. The Solution is Dilution. Upon completion of this lab, the student should be able to. • proficiently calculate molarities for solutions. • prepare a solution of known concentration. • prepare a dilute solution from a more concentrated one. • perform serial dilutions. • use volumetric and Mohr pipets and. Linearity determines the range over which lab tests are accurate and reportable. Serial Dilution - Download as Word Doc (.doc /.docx), PDF File (.pdf), Text File (.txt) or read online. Lab Report #9 Tifany Watson July 11, 2017 Serial Dilution and Pour Plate Technique Introduction: The objective of this lab is to learn the process of enumeration to determine the number of microorganisms in a sample. In certain for instances, the number of microorganisms in a sample over a period of time, is an important.
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